|
KMID : 1141020180180010001
|
|
Journal of Applied Oriental Medicine
2018 Volume.18 No. 1 p.1 ~ p.29
|
|
|
Effects of Ukgansan on the Rat Hypothyroidism Induced by PTU (6-n-propyl-2-thiouracil)
|
|
Park Gyu-Ryeul
Seo Bu-Il
|
|
|
Abstract
|
|
|
PURPOSE : The object of this study was to evaluate the effect of Ukgansan (UKS), aqueous extracts of mixed herbal formula consisted of nine herbs - Angelicae Gigantis Radix, Atractylodes Macrocephalae Rhizoma, Hoelen, Rhynchophylla Ramulusi, Cnidii Rhizoma, Bupleuri Radix, Glycyrrhizae Radix, Citri Pericarpium and Pinelliae Rhizoma on the PTU-induced rat hypothyroidism
METHODS : 6 groups, each of 8 rats per group were used in the present study as follows. Aqueous extracts of UKS were administered, once day for 42 days from 2 weeks before start of PTU treatment as an oral dose of 200, 100 and 50 ㎎/㎏ (body weight), and hypothyroidism was induced by daily subcutaneous treatment of PTU 10 ㎎/㎏ for 28 days. The changes on the body weight, thyroid gland, liver, epididymis and testis weights, serum thyroid hormone (TSH, T3 and T4) levels, serum male sex hormone (testosterone, DHT and FSH) levels, serum lipid profiles (total cholesterol, LDL, HDL and triglyceride), liver and testis antioxidant defense system (lipid peroxidation, H2O2, SOD and CAT), serum AST and ALT levels were observed with histopathology of thyroid gland, liver, epididymis and testis. Results were compared with LT4 0.5 ㎎/㎏ treated rats
Experimental groups : 6 groups (8 rats per groups were used) 1. Intact control : saline treated instead of PTU, and distilled water orally administered group 2. PTU control : PTU subcutaneously treated and distilled water orally administered group 3. LT4 : PTU subcutaneously treated and LT4 0.5 ㎎/㎏ intraperitoneally treated group 4. UKS200 : PTU subcutaneously treated and UKS extracts 200 ㎎/㎏ orally administered group 5. UKS100 : PTU subcutaneously treated and UKS extracts 100 ㎎/㎏ orally administered group 6. UKS50 : PTU subcutaneously treated and UKS extracts 50 ㎎/㎏ orally administered group.
RESULTS : As results of PTU treatment, marked decreases of body weights, increases of thyroid weights, liver, epididymis and testis weight decreases, serum T3, and T4 level decreases with increases of serum TSH levels, serum testosterone and DHT level decreases with increases of serum FSH levels, serum HDL increases and triglyceride content decreases, decreases of liver and testis antioxidant defense system, increases of serum AST levels were observed. In addition, marked hyperplasia of follicular cells with decreases of follicular colloid contents and sizes were demonstrated with decreases of hepatocyte numbers due to hypertrophy of hepatocytes and the increases of oligospermatic epididymal tubules at histopathological inspections. However, these PTU induced hypothyroidism were dose-dependently inhibited by treatment of UKS extracts, and UKS extracts effectively regulated the hypothyroidism related changes on the antioxidant defense system.
CONCLUSION : The results obtained in this study suggest that UKS extracts have favorable effects on the thyroid hormone productions with beneficial effects on the hypothyroidism related liver and reproductive organ damages mediated by the modulatory effects on the antioxidant defense system.
|
|
|
KEYWORD
|
|
|
Ukgansan (UKS), Hypothyroidism, PTU, 6-n-propyl-2-thiouracil
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|